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| Registros recuperados: 41 | |
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| Freire,D.A.C.; Oliveira,I.V.P.M.; Ferreira,H.I.P.; Andrade,V.C.P.; Silva,C.R.F.; Kurissio,J.K.; Ullmann,L.S.; Malossi,C.D.; Joaquim,S.F.; Araújo Júnior,J.P.; Langoni,H.; Calabuig,C.; Megid,J.; Antunes,J.M.A.P.. |
| RESUMO Doenças infecciosas são as maiores responsáveis por falhas reprodutivas (FR) em cadelas, causando aborto, morte fetal e natimortalidade. Este estudo teve como objetivo investigar a associação entre agentes infecciosos, FR inexplicáveis e anemia em cadelas. Todas as amostras maternas e fetais foram negativas para a presença dos principais agentes infecciosos causadores de FR: herpes vírus canino 1, Neospora caninum, Brucella spp. e B. canis, enquanto agentes como o de Leishmania spp., parvovírus canino, Ehrlichia canis e Anaplasma platys foram encontrados em sangue materno. Coinfecções de A. platys/E. canis e A. platys/Leishmania spp. foram diagnosticadas. Os resultados indicam que os animais com anemia causadas por doenças transmitidas por vetores... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Anaplasma; Ehrlichia; Leishmania; Reproductive failures. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501326 |
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| Diniz,Michely C.; Costa,Marcília P.; Pacheco,Ana C.L.; Kamimura,Michel T.; Silva,Samara C.; Carneiro,Laura D.G.; Sousa,Ana P.L.; Soares,Carlos E.A.; Souza,Celeste S.F.; Oliveira,Diana Magalhães de. |
| Several motile processes are responsible for the movement of proteins into and within the flagellar membrane, but little is known about the process by which specific proteins (either actin-associated or not) are targeted to protozoan flagellar membranes. Actin is a major cytoskeleton protein, while polymerization and depolymerization of parasite actin and actin-interacting proteins (AIPs) during both processes of motility and host cell entry might be key events for successful infection. For a better understanding the eukaryotic flagellar dynamics, we have surveyed genomes, transcriptomes and proteomes of pathogenic Leishmania spp. to identify pertinent genes/proteins and to build in silico models to properly address their putative roles in trypanosomatid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Actin-interacting proteins (AIPs); Flagellar proteins; Leishmania; Coronin and Arp2/3; Phagosome. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300033 |
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| Pinto,Erika G.; Tempone,Andre G.. |
| Abstract Background: Considering the high toxicity and limited therapies available for treating visceral leishmaniasis (VL), the drug repositioning approach represents a faster way to deliver new therapies to the market. Methods: In this study, we described for the first time the activity of a potent antiarrhythmic, amiodarone (AMD), against L. (L.)infantum and its in vitro and in vivo activity. Results: The evaluation against promastigotes has shown that amiodarone presents leishmanicidal effect against the extracellular form, with an IC50 value of 10 μM. The activity was even greater against amastigotes in comparison with promastigotes with an IC50 value of 0.5 μM. The selectivity index in relation to the intracellular form demonstrated that the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Visceral leishmaniasis; Amiodarone; In vitro; In vivo. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100320 |
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| Langoni,H.; Lucheis,S. B.; Da Silva,R. C.; Castro,A. P. B.; Paes,A. C.. |
| Visceral leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus. Dog is the major source of infection to man, especially in urban areas. The authors report a case of visceral leishmaniasis in a pit bull female dog from Bocaina, São Paulo, Brazil. The animal presented clinical signs compatible with leishmaniasis, including skin lesions in the body and partial damage of the external ears. The indirect fluorescent antibody test (IFAT) demonstrated a titer of 1280, and promastigote forms of Leishmania sp were isolated by the culture of bone marrow puncture. Cytological analysis of the lymph node and smear of the bone marrow puncture revealed macrophages containing amastigote forms of Leishmania sp in their inner region. The test of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Zoonosis; Dog; Diagnosis; Hemoculture; Serology; Polymerase chain reaction (PCR). |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300012 |
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| Napolitano,H.B.; Silva,M.; Ellena,J.; Rodrigues,B.D.G.; Almeida,A.L.C.; Vieira,P.C.; Oliva,G.; Thiemann,O.H.. |
| Several natural compounds have been identified for the treatment of leishmaniasis. Among them are some alkaloids, chalcones, lactones, tetralones, and saponins. The new compound reported here, 7-geranyloxycoumarin, called aurapten, belongs to the chemical class of the coumarins and has a molecular weight of 298.37. The compund was extracted from the Rutaceae species Esenbeckia febrifuga and was purified from a hexane extract starting from 407.7 g of dried leaves and followed by four silica gel chromatographic fractionation steps using different solvents as the mobile phase. The resulting compound (47 mg) of shows significant growth inhibition with an LD50 of 30 µM against the tropical parasite Leishmania major, which causes severe clinical manifestations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coumarin; Aurapten (7-geranyloxycoumarin); X-rays; Leishmania; Inhibitor. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200010 |
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| Sobreira Dantas Nóbrega de Figuêiredo,Francisco Rodolpho; Gyllyandeson,de Araújo Delmondes; Paulo da Cruz,Luzia; Amaro do Nascimento,Érika; Relison Tintino,Saulo; Santiago Lemos,Izabel Cristina; Bezerra da Cunha,Francisco Assis; Vega Gomez,Maria Celeste; Coronel,Cathia; Martins da Costa,José Galberto; Melo Coutinho,Henrique Douglas; Bezerra Felipe,Cícero Francisco; Alencar de Menezes,Irwin Rose; Kerntopf,Marta Regina. |
| As doenças parasitárias, também chamadas de “doenças negligenciadas”, continuam sendo uma grande dificuldade para o desenvolvimento social e econômico dos países mais pobres. Podemos citar como exemplo dessas doenças, a leishmaniose e a doença de Chagas. A leishmaniose é causada por parasitas do gênero Leishmania e afeta cerca de 12 milhões de pessoas. A doença de Chagas, causada pelo protozoário Trypanosoma cruzi, causa aproximadamente 50.000 mortes por ano. Os fármacos disponíveis para o tratamento dessas doenças são altamente tóxicos, sendo este um dos motivos que leva à busca por drogas eficazes e seguras para seus tratamentos. As folhas da Annona squamosa, espécie da família Annonaceae, já foram descritas na literatura por suas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Annona squamosa; Annonaceae; Leishmania; Trypanosoma cruzi. |
| Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-37432016000300001 |
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| Godoy,K.C.S.; Braz,P.H.; Assis,A.R.; Antunes,T.R.; Gomes,D.C.; Souza,A.I.. |
| O objetivo deste estudo foi avaliar os indicadores laboratoriais, eletrocardiográficos e histológicos de lesão cardíaca em diferentes grupos clínicos de cães com leishmaniose visceral. Foram analisados marcadores séricos, traçado eletrocardiográfico e fragmentos de tecido cardíaco de 41 cães naturalmente infectados, distribuídos em três grupos: assintomático, oligossintomático e sintomático. Todos os animais apresentaram aumento na atividade sérica da enzima creatina quinase fração MB. No traçado eletrocardiográfico, o complexo de baixa voltagem foi o distúrbio de condução mais frequente (8/10). Na análise histológica, 75,6% dos cães apresentaram reação inflamatória com predomínio de infiltrados linfo-histiocítico (13/31) de intensidade discreta a moderada... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cardiomiopatia; Enzimas; Leishmania; Marcadores bioquímicos; Miocardite. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200313 |
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| Oliveira,Diego Molina de; Lonardoni,Maria Valdrinez Campana; Teodoro,Ueslei; Silveira,Thais Gomes Verzignassi. |
| OBJECTIVE: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous leishmaniasis (ACL) diagnosis. MATERIAL AND METHODS: Five pairs of primers, four of them derived from Leishmania kDNA sequences (MP3H-MP1L; B1-B2; LBF1-LBR1; 13A-13B), and one derived from the SL RNA (mini-exon) gene repeat (LU5A-LB3C), reported previously, were used. RESULTS: The MP3H-MP1L primers were the best at amplifying the DNA, detecting 2 fg of Leishmania spp. DNA. The 13A-13B primers presented the worst performance, detecting 512 x 10³ fg of DNA. CONCLUSION: The wide variation in the analytical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmaniasis; Polymerase chain reaction; Leishmania; DNA primers. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300004 |
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| Teixeira,S.M.R.. |
| The study of mechanisms which control gene expression in trypanosomatids has developed at an increasing rate since 1989 when the first successful DNA transfection experiments were reported. Using primarily Trypanosoma brucei as a model, several groups have begun to elucidate the basic control mechanisms and to define the cellular factors involved in mRNA transcription, processing and translation in these parasites. This review focuses on the most recent studies regarding a subset of genes that are expressed differentially during the life cycle of three groups of parasites. In addition to T. brucei, I will address studies on gene regulation in a few species of Leishmania and the results obtained by a much more limited group of laboratories studying gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma; Leishmania; Gene expression; RNA; Antigenic variation; VSG. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200001 |
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| Teodoro,Ueslei; Galati,Eunice A.B.; Kühl,João Balduíno; Lozovei,Ana Leuch; Barbosa,Orlando Carlos. |
| Evaluation of DDT insecticide used to sand flies control in domiciles and domestic animal shelters in an endemic area of cutaneous leishmaniasis. Collections of sand flies were made with Falcão traps in houses, in one pigsty and in one tree in Jussara Farm, municipality of Jussara, State of Paraná, Brazil, between 10 p.m. and 2 a.m. during 7 days from March 24 to April 1, 1992. In May, 1992 DDT insecticide (Dicloro Difenil Tricloro Etano) (1 g/square meter of area) was applied internally and externally on houses and in pigsty. After this, new collections of sand flies were made from July 1992 to February 1993, once a month, with Falcão traps installed in the same hour and conditions mentioned above. Before DDT insecticide application 12,960 sand flies were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Leishmaniose; Controle de flebotomíneos; DDT. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300014 |
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| Takahashi,Helio K.; Toledo,Marcos S.; Suzuki,Erika; Tagliari,Loriane; Straus,Anita H.. |
| Recently, glycosphingolipids have been attracting attention due to their role on biological systems as second messengers or modulators of signal transduction, affecting several events, which range from apoptosis to regulation of the cell cycle. In pathogenic fungi, glycolipids are expressed in two classes: neutral monohexosylceramides (glucosyl-or galactosylceramide) and acidic glycosylinositol phosphorylceramides (the latter class carries longer glycan chains). It is worth to mention that monohexosylceramides exhibit significant structural differences in their lipid moieties compared to their mammalian counterparts, whereas the glycosylinositol phosphorylceramides exhibit remarkable structural differences in their carbohydrate moieties in comparison to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycosphingolipids; Glycosylinositol phosphorylceramides; Inositol phosphorylceramide; Leishmania; Membrane rafts; Pathogenic fungi. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300012 |
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| Barbosa,V.T.; Silva,M.A.G.; Sousa,M.G.; Gering,A.P.; Santos,H.D.; Laus,J.L.. |
| Leishmaniose é uma enfermidade multissistêmica cujas manifestações clínicas são extremamente variáveis. Em cães sinais clínicos oftálmicos são relativamente frequentes, ainda que outros sinais sistêmicos não sejam identificados. Atualmente, o diagnóstico da doença baseia-se em métodos parasitológicos, sorológicos e moleculares, mas, até o momento, a identificação de formas amastigotas desse parasito em esfregaços feitos a partir de suabes conjuntivais não é empregada rotineiramente. Valendo-se de cães sorologicamente positivos para leishmaniose, portadores (G1) ou não (G2) de alterações oftálmicas, este estudo avaliou a viabilidade do esfregaço a partir de suabe conjuntival como método de diagnóstico para a enfermidade. O exame suprarreferido foi positivo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cão; Leishmania; Linfonodo; Conjuntiva. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600009 |
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| Romão,P.R.T.; Tovar,J.; Fonseca,S.G.; Moraes,R.H.; Cruz,A.K.; Hothersall,J.S.; Noronha-Dutra,A.A.; Ferreira,S.H.; Cunha,F.Q.. |
| Glutathione is the major intracellular antioxidant thiol protecting mammalian cells against oxidative stress induced by oxygen- and nitrogen-derived reactive species. In trypanosomes and leishmanias, trypanothione plays a central role in parasite protection against mammalian host defence systems by recycling trypanothione disulphide by the enzyme trypanothione reductase. Although Kinetoplastida parasites lack glutathione reductase, they maintain significant levels of glutathione. The aim of this study was to use Leishmania donovani trypanothione reductase gene mutant clones and different Leishmania species to examine the role of these two individual thiol systems in the protection mechanism against S-nitroso-N-acetyl-D,L-penicillamine (SNAP), a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Glutathione; Trypanothione; Trypanothione reductase; Nitric oxide; Free radicals. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300006 |
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| Camargo,L. B; Langoni,H.. |
| Leishmaniasis is a parasitic zoonosis caused by protozoans of the genus Leishmania transmitted by insects known as phlebotomines, which are found in wild or urban environments. It affects domestic and wild animals and transmission to man happens by accident. The disease occurs in tropical and sub-tropical areas, mainly in Asia, Europe, Africa, and the Americas. There are two forms that affect man: American cutaneous leishmaniasis (ACL) and American visceral leishmaniasis (AVL). The latter is caused by three species of Leishmania: Leishmania (Leishmania) donovani, Leishmania (Leishmania) infantum, and Leishmania (Leishmania) chagasi, which are grouped in the Leishmania (Leishmania) donovani complex. Wild reservoir hosts of L. chagasi known so far are foxes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmaniasis; Cutaneous leishmaniasis; Visceral leishmaniasis; Leishmania. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400002 |
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| Registros recuperados: 41 | |
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